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KU Nuclear Magnetic
Resonance Laboratory
The Nuclear Magnetic Resonance Laboratory is
responsible for maintaining the high field NMR spectrometers, training
users, providing spectra on a service basis, and assisting users with
design, execution, and
interpretation of NMR experiments. Our capabilities extend from small
molecules
to isotopically enriched proteins, solids, and flow samples. We are
part
of the Molecular
Structures
Group (MSG) at KU, a campuswide facility encompassing NMR, MS,
X-ray
Crystallography, Biochemical Service, and Molecular Graphics and
Modeling
Laboratories. We manage seven shared NMR instruments:
Structural Biology Center and 800 MHz NMR. The NMR Lab
received
an 800 MHz instrument in September 2004 (and equipped it with a TCI
cryoprobe in September 2005), housed in a specialized
laboratory
in room 100 of the new Structural
Biology Center (SBC). We share this building with the Protein
Structure Lab (a new MSG unit devoted to protein X-ray
crystallography), a satellite Molecular Graphics and
Modeling Lab, and the Analytical Proteomics Lab (a joint
venture of the Mass Spectrometry
and Biochemical Research Service Labs), in a second phase of the SBC
which opened in December 2004.
(For more information about the Structural Biology Center and our
scientific goals for this new facility, click here.
Pictures from the 800 MHz installation are here.)
The third phase of Structural Biology will open in June 2008, housing
the Center of Excellence in Chemical
Methodologies and Library Development (CMLD), and KU's High Throughput Screening Lab. A
fourth phase of SBC is under construction.
In addition to the 800, we also have two Avance 400's in 104
SBC, one with an
H/C/P/N QNP gradient probe, and one with a broadband gradient
probe. The second
instrument, part of
KU's NIH-funded
center of excellence in Chemical Methodology and Library Development
is equipped with a 24 position
NMR-CASE sample changer for conventional NMR tubes. It also has BBI
and FI gradient probes, and a flow injection BEST system. We have
also implemented the ERETIC experiment under automation with this
system, for automated determination of absolute solution concentration
with the sample changer.
A third Bruker DRX-400 with a H/C/P/F QNP gradient probe in
3002
Malott Hall is
managed principally for self-service rapid turnaround of routine
samples. Most graduate students whose projects
involve synthesis or chemical characterization are trained to use one
or
more of the 400 instruments early in their studies. Accessibility of
these
instruments is excellent (ordinarily the same day the sample is
prepared).
In addition to the host workstations on the instruments, the lab also
houses
three PC's running Bruker's NMR Suite, plus other Linux and Windows
software. The
3002 lab also contains a walkup GC-MS instrument with an autosampler,
managed by the Mass Spectrometry Laboratory.
In B042 Malott, we have a three channel Bruker DRX 500 with
broadband, inverse triple resonance, and high resolution magic angle
spinning (HR-MAS) probes, and an NMR-CASE sample changer. The
instrument also has an MAS controller, a modified BCU-05 temperature
bath for low temperature MAS operation, and a liquid nitrogen boiloff
variable temperature controller. The B042 lab also houses an Avance
AV-III 500 with a dual
carbon/proton (CPDUL)
cryoprobe and a 60 position BACS sample changer. This instrument is
used
primarly under ICON-NMR automation and PDF plot files and binary data
can be emailed directly to the requestor as soon as the experiments are
finished. Our 800 and three cryo/cold probes gives us one of the
best-equipped and most modern NMR
facilites in the region. All of the spectrometer host
workstations have been
upgraded to RedHat Enterprise Linux
4. The Bruker AV instruments run TopSpin 2.1 and the DRX
instruments run TopSpin 1.3.
A four-channel Varian Inova 600 MHz system
suitable for nD experiments on
proteins is located in room 102 of the Multidisciplinary Research
Building. It has triple resonance XYZ gradient and broadband Z gradient
probes. We received a triple resonance PFG cold probe for this
instrument in July 2005. The data system was
updated to
VnmrJ LX 2.1B/Red Hat Enterprise Linux 4.
Information of interest to local users, such as training
guides, sample submission forms, and lab policies, can be found on our
user pages at http://kunmr-suse.msg.ku.edu.
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